Tissue Sectioning Methods
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Your tissue section quality and its integrity is only as good as your fixation technique.
Tissue sectioning methods. The tissue is typically cut into thin sections 5 10 µm or smaller pieces for whole mount studies to facilitate further study. Older browsers that do not support html5 and the h 264 video codec will still use a flash based video player. If your sample is difficult to section because the tissue itself is hard think.
Using the anti roll plate. Apply tissue on the coated slide followed by adding a drop of mounting medium glycerol gelatin on the tissue hold the coverslip at 45 allowing the drop to spread along the edge of the slip slowly cover the tissue entirely with the coverslip incubate the slide at 80 for 1 hour if there is an adhering problem with a tissue section. Embedding is important in preserving tissue morphology and giving the tissue support during sectioning.
The cutting and floating method for paraffin embedded tissue for sectioning the jove video player is compatible with html5 and adobe flash. Then a quick overview of mhpl cryostat sectioning techniques. Preparing tissue for freezing.
Some epitopes may not survive harsh fixation or embedding. How to section difficult tissues. After fixation tissue specimens are routinely embeddedin a solid material which will support very thin sectioning.
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