Tissue Sectioning

Fill a waterbath with ultrapure water and heat to 40 45 o c.

Tissue sectioning. It is important to have a properly fixed and embedded block or much artefact can be introduced in the sectioning. Both fresh and fixed tissues can be processed as frozen tissues. If your sample is difficult to section because the tissue itself is hard think.

The clearance angle prevents. Histotechnologists are the artists of the laboratory. Cold wax allows thinner sections to be obtained by.

Cryosectioning frozen sections tissues will be cut at 5 microns unless otherwise indicated on form. Bring your own slide boxes or purchase from core. In the histology laboratory conventional tissue processing describes the stages required to take fixed tissue samples through dehydration and clearing to the state where it is completely infiltrated and embedded with a suitable medium normally paraffin wax in readiness for cutting sections on a microtome microtomy.

Paraffin embedded tissue when generating paraffin embedded tissue samples the tissue must be fixed before embedding in paraffin. How to section difficult tissues. Sections will always get lost during sectioning but it is imperative that it be noted where the tissue was lost and how much was lost.

If more tissue than that is lost at any place in a ribbon then a note should be made under sectioning comments. Sections are placed on superfrost plus slides charged. The tissue is typically cut into thin sections 5 10 µm or smaller pieces for whole mount studies to facilitate further study.

Sectioning slicing provides the very thin specimens needed for microscopy. With proper treatment the section reveals clear tissue structure and exact antigen location to enable high medical value pathology researches and retrospective studies. One section per slide unless otherwise requested.